Evaluasion Of Low Temperature Induced Mutant Of Soubean Mosaic Virus For Cross Protection In Soybean

Early natural infection by soybean mosaic virus (SMV) can reduce seed production in soybean. Cross protection enables the production of SMV free seed and is a mechanism which can signficantly reduce the impact of SMV. We proposed that attenuated isolates of SMV obtained by cold temperature treatments are able to produce SMV free seed in soybean [Glycine max (L.)] Merr. plants. We inoculated the cotyledons with SMV infected plants, incubated at low temperature, mechanically inoculated seedlings with virus were subjected to cold and finally transplanted into pot and then into the field. We examined plants with different symptoms. Serological assays, RT-PCR analysis and electron micrography did not distinguish between the very mild mosaic symptom of attenuated isolate and the original virulent isolate. However, the mutant of SMV did not give rise to local lesions on Chenopodium amaranticolor. Our results suggest that attenuated isolate of SMV is potentially useful for reducing the impact of SMV infection. When plants are inoculated with the attenuated isolate at 8 days after planting, symptoms of the disease do not develop.

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